Primary

• Mito Uzumaki Grandmother of Tsunade
• Mito Uzumaki Grandmother of Nawaki
• Mito Uzumaki Wife of Hashirama Senju
• Mito Uzumaki Sister-in-law of Tobirama Senju

Extended

• Tsunade Granddaughter of Hashirama Senju
• Tsunade Lover of Dan Katou
• Tsunade Granddaughter of Mito Uzumaki
• Tsunade Grandniece of Tobirama Senju
• Tsunade survival fight against Hanzo of the Salamander
• Tsunade Former teammate of Jiraiya
• Tsunade Former teammate of Orochimaru
• Tsunade Teacher of Sakura Haruno
• Tsunade Student of Hiruzen Sarutobi
• Tsunade Older Sister of Nawaki
• Nawaki Younger Brother of Tsunade
• Nawaki Grandson of Hashirama Senju
• Nawaki Grandson of Mito Uzumaki
• Hashirama Senju Grandfather of Tsunade
• Hashirama Senju Older Brother of Tobirama Senju
• Hashirama Senju Grandfather of Nawaki
• Hashirama Senju Friend of Madara Uchiha
• Hashirama Senju Husband of Mito Uzumaki
• Hashirama Senju reincarnation of Asura Otsutsuki
• Hashirama Senju held the first meeting of the kages with Ishikawa
• Hashirama Senju held the first meeting of the kages with Byakuren
• Hashirama Senju Genetic Template of Zetsu
• Hashirama Senju Genetic Template of Yamato
• Tobirama Senju Younger Brother of Hashirama Senju
• Tobirama Senju Uncle of Tsunade
• Tobirama Senju former teacher of Hiruzen Sarutobi
• Tobirama Senju former teacher of Homura Mitokado
• Tobirama Senju former teacher of Koharu Utatane
• Tobirama Senju really hates Orochimaru
• Tobirama Senju Co-worker of Minato Namikaze
• Tobirama Senju Enemy of Madara Uchiha
• Tobirama Senju Enemy of Izuna Uchiha
• Tobirama Senju Brother-in-law of Mito Uzumaki