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That's Sergeant Keroro, from ＂Sergeant Frog＂ (original title ＂Keroro Gunsou＂). He's the protagonist, and seems to be a total king - he's got a 358-episode anime series and several movies under his belt. Not bad.

Here's his ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=64355

Ereshkigal from fate/grand order!

This is a vtuber named Filian!

That's the Protagonist from Persona 3, as he appears in Persona 3 Reload. Fans typically refer to him as Minato Arisato or Makoto Yuki (names taken from spin off media where he needs to have a name) for simplicity. Actually, on checking things out, it seems Makoto Yuki has in fact been made his canon name.

Here's his ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=19294

The image source is his official character art for Persona 3 Reload. You can source it from the MegaTen Wiki as one example: https://megatenwiki.com/wiki/File:P3R_Protagonist_Artwork.png

Oh that looks beautiful. I'm definitely giving that artist a follow on Pixiv. Thanks, Natsumi.

I found the image!

https://danbooru.donmai.us/posts/8771505

Original character.

Source: https://www.pixiv.net/en/artworks/26018696

Henya the Genius

That's Shotaro Kaneda, protagonist of Akira

Here's his ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=17846

That's Rutile, from Houseki no Kuni (localized as ＂Land of the Lustrous＂).

Here's her ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=85437

I recognized that design instantly. That's Asuka from Senran Kagura. I'm unable to find the original source of the image which is a bit weird, but that's definitely her.

Here's her ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=53276

That's Dorothea, from Fire Emblem Three Houses.

Here's her ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=122623

It's a guess, but it might me Kurara Amamiya

https://www.animecharactersdatabase.com/characters.php?id=14216

Or Harugasaki Kanai

https://www.animecharactersdatabase.com/characters.php?id=98030

It could be original as well

Hunter x Hunter ep. 75

Lucy from Fairy Tail

Aqua from Kingdom Hearts

Phyllis and Garu from I was reincarnated as a blind girl

Full image: https://danbooru.donmai.us/posts/2350721

The original tweet was deleted, but it's by Kakutasu

Hsien-Ko from a fighting game series called Darkstalkers

This is a vtuber named Mint Fantôme! She used to be under the vshojo agency, but she's an independent vtuber now

https://snipboard.io/T2DXpx.jpg

Sorry, better quality image here

Lily from Monster no Goshujin-sama.

It's from Chapter 50

is from Naruto Shippuden Episode 165

Nice. I didn't even consider looking here. Kinda surprised at myself. XD

We have vtubers here as well.

https://www.animecharactersdatabase.com/characters.php?id=117590

https://myanimelist.net/anime/47591/Kuro_Gal_ni_Natta_kara_Shinyuu_to_shitemita/characters

The blondes are the same people called Shion. Black haired is Rui

That's Kongou, from Kantai Collection.
Here's her ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=70472

That's Pomu Rainpuff, formerly a vtuber for Nijisanji EN, who has since retired and eventually came back in indie form as Mint Fantôme.

Since this specific character graduated, she no longer has a page on the official site, so instead I'll give you her Virtual Youtuber Wiki entry. https://virtualyoutuber.fandom.com/wiki/Pomu_Rainpuff

Right is Wei Si Ji from Travel to AD 3000

Here is the full image

Left is Xiangnuan from Prince, you are so cheap and right is Zheng Zha from Terror Infinity

Harley Quinn Vol. 2: Power Outage The New 52

https://dc.fandom.com/wiki/Harley_Quinn:_Power_Outage_(Collected)

Cantarella from Wuthering Waves

That's Gwenpool, a Marvel character. She has her own Wikipedia page if you're interested: https://en.wikipedia.org/wiki/Gwenpool

The image source is an animation by Spineapples. He uploaded the animation to Youtube himself; ＂Gwenpool gamer rage＂, fitting title indeed.

LOUD AUDIO WARNING: https://youtu.be/yvRMTQtvVII

It's also full of swears, not that I'm a stranger to swearing myself but just in case you are.

The artist is jivke. Apparently they've posted the art themselves on Twitter, and the link to that post would be this one: https://x.com/jivke/status/1257088598869827584

However it gives me a ＂this page doesn't exist＂ notice. Maybe it's because the account is marked as being too NSFW for unregistered users like me to see. On the off chance they did actually delete the post, you can still find it hosted on danbooru: https://danbooru.donmai.us/posts/3908507

Of course, take care browsing most booru sites since you know what these are mostly for.

That's Ceres Fauna, a vtuber for Hololive. Here's her page on the official Hololive website: https://hololive.hololivepro.com/en/talents/ceres-fauna/

The image source is fanart drawn by Ginotty; you can find an official upload of the art on Twitter: https://x.com/ginotty/status/1861384940098855046

That's Anos Voldigoad, protagonist of ＂The Misfit of Demon King Academy＂!

Here's his ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=103380

Yuu Ayase from ＂Mikadono Sisters Are Surprisingly Easy＂ (the anime of the series calls it ＂Dealing with Mikadono Sisters Is a Breeze＂)

Mashiro Shiina from the anime/manga ＂The Pet Girl of Sakurasou＂

Yuri from DDLC

Image source is the game

Simon, aka Simon the Digger, from the Gurren Lagan series!

That's Sukuna, from Jujutsu Kaisen.

Here's his ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=104853

That's Ty Rux, the protagonist of Dinotrux - an animated show that originally came out on Netflix.

https://en.wikipedia.org/wiki/Dinotrux

That's Mob - real name Shigeo Kageyama.

Here's his ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=77822

That's Rikka Hishikawa from Doki Doki PreCure.

Here's her ACDB entry! https://www.animecharactersdatabase.com/characters.php?id=58241

That's Otome Saotome, from Shimoneta.

Here's her ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=73907

That's Ikuyo Kita from Bocchi the Rock, and the image source is a piece of fan art from kotatsu-mazoku on Pixiv.

Here's her ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=126392

And this is the original Pixiv upload of that artwork: https://www.pixiv.net/en/artworks/120263473

https://netflix.fandom.com/wiki/The_Red_Bluster

Red

Nishikata from Karakai Jouzu no Takagi-san

Transformers

Itachi and Sasuke Uchiha

Kaname Tohyama

https://hidannoaria.fandom.com/wiki/Kaname_Tohyama

The original source for the image is gone but it's AI by rouga_aiart.

Starting from the left, I can't 100% confirm who the first two characters are. However, the last two are certainly Hasaki Mamiya and Yuki Minakami from ＂Wonderful Everyday ~Discontinuous Existence~＂. I would not be surprised if the first two are also characters from the same work.

Here's the ACDB entries of the characters I did confirm:
Hasaki Mamiya - https://www.animecharactersdatabase.com/characters.php?id=21103
Yuki Minakami - https://www.animecharactersdatabase.com/characters.php?id=21109

Here's the list of characters that the site has entries for regarding this series, in case you're curious: https://www.animecharactersdatabase.com/source.php?id=2363&tab=Characters

Those are Shiu Kong on the left, and Toji Fushiguro on the right. They are characters from Jujutsu Kaisen.
Here you have their ACDB entries.

Shiu Kong: https://www.animecharactersdatabase.com/characters.php?id=131309
Toji Fushiguro: https://www.animecharactersdatabase.com/characters.php?id=131310

Those are characters from Mob Psycho. They make up the Body Improvement Club. And seeing them reminds me I have to work out.
From left to right, here's their ACDB entries.

Hideki Yamamura: https://www.animecharactersdatabase.com/characters.php?id=104214
Jun Sagawa: https://www.animecharactersdatabase.com/characters.php?id=104216
Hiroshi Kumagawa: https://www.animecharactersdatabase.com/characters.php?id=104218
Musashi Gouda: https://www.animecharactersdatabase.com/characters.php?id=77828
Ryohei Shimura: https://www.animecharactersdatabase.com/characters.php?id=104217

Those are Garp and Aokiji from One Piece. Here's their ACDB entries.

Garp: https://www.animecharactersdatabase.com/characters.php?id=27177

Aokiji (real name ＂Kuzan＂): https://www.animecharactersdatabase.com/characters.php?id=27172

The image is from this scene of theirs in episode 1121: https://youtu.be/Yp1yHJTSXDA

Hey this looks really cool! I might just give it a shot and read it. The source of that image is ＂Kou-1 desu ga Isekai de Joushu Hajimemashita＂. In English, it got localized as ＂I'm a High Schooler, But I Became an Owner of a Castle in Another World＂. That image is specifically the last page for chapter 2 of the manga.

My Anime List has an entry for the series, check it out here: https://myanimelist.net/manga/94813/Kou-1_desu_ga_Isekai_de_Joushu_Hajimemashita

As for the characters, they are the main male and female protagonists - Hiroto Kiyokawa and Valkyria. They have no really useful entries anywhere I've seen, so you'll have to have their Kou-1 Fandom Wiki pages instead.

Hiroto Kiyokawa: https://kou1isekai.fandom.com/wiki/Hiroto_Kiyokawa

Valkyria: https://kou1isekai.fandom.com/wiki/Valkyria

That's Kuro Kurenai, a VShojo vtuber. Here's his page on the VShojo website: https://www.vshojo.com/talent/kuro-kurenai

I assume you mean the two characters that the page puts the emphasis on. They are Hitomi Takano (left) and Angelica Karasuma (right) from Hitomi-chan wa Hitomishiri (localized as ＂Hitomi-chan Is Shy With Strangers＂). That page is from Chapter 61 of the manga.

You can find entries for the two of them over at My Anime List:

Hitomi Takano: https://myanimelist.net/character/170485/Hitomi_Takano

Angelica Karasuma: https://myanimelist.net/character/222493/Angelica_Karasuma

That's Marin Kitagawa from My Dress-Up Darling. She's cosplaying as Liz-kyun (or Rizu-kyun), a character from a manga series that exists only in-universe (a fictional work within a fictional work).

Here's their ACDB entries.
Marin Kitagawa: https://www.animecharactersdatabase.com/characters.php?id=115747
Rizu-kyun: https://www.animecharactersdatabase.com/characters.php?id=123295

Kaoruko Waguri

Midori Arisugawa and Ryuu Yamada from Yamada-kun to 7-nin no Majo

Source: https://www.pixiv.net/en/artworks/72942020 but you have to log in due to it being NSFW.

Otherwise, there's gelbooru: https://gelbooru.com/index.php?page=post&s=view&id=4592714

Aiko Torasawa from Torako! Don't Break Everything!

It's from chapter 21

Lumi from Parallel Paradise

https://myanimelist.net/character/153332/Lumi

It's from chapter 2

Original is Shinka Nibutani. Someone just painted over it

Source: https://www.pixiv.net/en/artworks/31543061

Marin Kitagawa

Anubis by Houtengeki

Source: https://www.pixiv.net/en/artworks/39930955

Anzu Hoshino

Inami and Masato from Kamatte Shinsotsu-chan ga Maikai Sasottekuru

That's Cookie 4 (as in, Cookie's 4th form) from Majikoi S (full name ＂Maji de Watashi ni Koi Shinasai! S＂).

This specific form for the character does not have an page in here. Instead, have her VNDB entry: https://vndb.org/c56722

Those are Marcy Wu and Anne Boonchuy, from Amphibia. That's a screenshot from one of the episodes - ＂The Sleepover to End All Sleepovers＂. For whatever reason, there's a very convenient Twitter post that exists to source this: https://x.com/Amphibia_frames/status/1767747461567684608

Here's their ACDB entries.
Marcy Wu: https://www.animecharactersdatabase.com/characters.php?id=122698
Anne Boonchuy: https://www.animecharactersdatabase.com/characters.php?id=122699

Full image source: https://www.pixiv.net/en/artworks/63292071

The characters are Fujiwara no Mokou and Kaguya Houraisan from Touhou. Here you have their ACDB entries:

Fujiwara no Mokou: https://www.animecharactersdatabase.com/characters.php?id=20162

Kaguya Houraisan: https://www.animecharactersdatabase.com/characters.php?id=20161

This image seems to have quite the history! It's the icon for Dynasty Scans, a scanlation group with their own online reader and everything.

The source of the image is a Touhou comic by an artist named ＂Ayakashi＂, and it's hosted in their site too. This is a link to that specific page - site seems harmless security wise but as always, browse with care: https://dynasty-scans.com/images/4551

That's Elphelt Valentine from Guilty Gear. Here's her ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=83560

That specific image is fanart, made by Finalcake - a digital artist, and quite the good one from what I can see.

That's Ami Uehara, as seen in Love Selection: The Animation. Its author seems to have made a bunch of interesting stuff in general.

She has an entry in MyAnimeList: https://myanimelist.net/character/37151/Ami_Uehara

I was wondering why that seemingly safe image was marked as NSFW, then I saw the original gif. ^^

That's Nao Iihara from Boin. Here's her ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=1010

Lucky Girl

Seems like it's Yuuki Shibuki, the protagonist of ＂Miageru to Kimi wa＂ (referred to as ＂Looking Up to You＂ in English).

He doesn't have an entry on here, but he does have one at MyAnimeList: https://myanimelist.net/character/171050/Yuuki_Shibuki

That's Gigi Murin, a Hololive character

She has an entry in ACDB, too: https://www.animecharactersdatabase.com/characters.php?id=140885

I remember this one! That's a screenshot from Seikon no Qwaser (＂The Qwaser of Stigmata＂ in English). The character is Urara Oikawa.

Here's her ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=34351

That's Will Vandom, from W.I.T.C.H.

Here's her ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=115693

On the left it's Penny Polendina from RWBY, on the right it's Yoichi Isagi from Blue Lock.

Penny: https://www.animecharactersdatabase.com/characters.php?id=89116

Yoichi: https://www.animecharactersdatabase.com/characters.php?id=117087

Indeed. Specifically, it's her official art from Persona 5: Dancing in Starlight.

Here's her ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=77625

Maomao and Jinshi from The Apothecary Diaries

Tomoe Futaba from Futsal Boys!!!!!

Black Swan from Saint Seiya

Fringilla Vigo from The Witcher

Ann Takamaki from Persona 5

Image source was official game

Yeah there's no doubt in my heart that's Sakuya Izayoi. I was about to say that yesterday but that exact picture they link to is unfindable.

That's Morgiana, from Magi: the Labyrinth of Magic.

She has an ACDB entry, too: https://www.animecharactersdatabase.com/characters.php?id=47218

Mikoto Urabe, from Nazo no Kanojo X (Mysterious Girlfriend X).

Here's her entry on the site: https://www.animecharactersdatabase.com/characters.php?id=44021

That's Escanor from Seven Deadly Sins. Here's his ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=99287

Aha, you know it's a good design when even I am this quick to recognize it. That's Gabriel White Tenma from ＂Gabriel Dropout＂. Here's her ACDB entry: https://www.animecharactersdatabase.com/characters.php?id=78430

I'm not sure, but it looks like Sakuya Izayoi to me

Philuffy Aingram, Lux Arcadia and Yoruka Kirihime from Saijaku Muhai no Bahamut

Yang Xiao Long

https://x.com/princesshinghoi/status/1214520965339598848

Yuki Nagato

Left is Sakurako Oomuro https://www.animecharactersdatabase.com/characters.php?id=26357
Right is Himawari Furutani https://www.animecharactersdatabase.com/characters.php?id=26353
From the Yuru yuri series

Kiyomi Aramiya, Kotoko Ayame, Yuuka Hatsushiba, Ryouma Saitani and Eve Suwama