Nicknamed Riku, she is a general serving under Nobuna. Officially, she is an aide to Nobuna's younger brother Nobukatsu but her true loyalty lies to Nobuna.

Source:Wikipedia

The main female protagonist and the Daimyo of Owari.

She has a tsundere attitude and is known by her enemies as the "Fool of Owari". She is a brilliant and ambitious teenage girl who plans to conquer and unite Japan, to make it a strong nation equal to the Europeans.
As an admirer of foreign culture and items, their influences inspires Nobuna's ambition.

Source:Wikipedia

The main male protagonist.
A modern-day teenager who finds himself in an alternative version of the Sengoku period where some of the famous warlords of the era are female. Using his knowledge from the videogame, Nobunaga's Ambition, Yoshiharu helps Nobuna in her quest to unite and conquer Japan.

He also goes by the nickname "Monkey", that Nobuna gives him, much to his chagrin.

Source:Wikipedia

Position: Offensive Guard / Defensive Tackle

Komusubi is one of the linemen of the Deimon Devil Bats. Though Daikichi is not very fast nor tall, he makes up for it with his indomitable will and power. He decided to join the Devil Bats after highly impressed by Kurita's amazing strength and build, and decided that he wanted to become Kurita's "apprentice".

Source:Wikipedia

Position: Cheerleading Captain (self-appointed)

Suzuna is a cute blue-haired girl who's always wearing rollerblades.
She came to the United States to look for her brother and hitched a ride with one of the bikers of a bike gang.

Later, after her brother accepts Sena's offer to join the Devil Bats, Suzuna follows the team back to Japan. She then becomes a cheerleader for the Devil Bats.

In the anime, Suzuna was introduced earlier in the timeline as a part-time reporter, working for the magazine American Football Monthly to discover Eyeshield 21's identity. Her reason was to find her long-lost brother, whom she suspected was Eyeshield 21. Realizing that Eyeshield 21 was too short however, she then leaves for America due to a tip from Hiruma.

Source:Wikipedia

Sena's overprotective and motherly childhood friend and later, the manager of the team.

Mamori's mother met Sena's mother in a cooking class and Mamori and Sena have been friends ever since. Mamori has been protecting Sena from bullies since primary school, to the point that Sena has grown up never having to stand up for himself.

She joined the American Football club to watch over Sena and prevent any bullying by the other members (particularly Hiruma). Despite her joining the American Football club just to protect Sena, she eventually becomes an excellent manager who knows the rules inside and out and makes up strategies for the team.

She is also a member of the school's disciplinary committee.

Source:Wikipedia

Position: Wide Receiver / Split end / Cornerback

Monta was originally a member of the baseball team, where he was an effective fielder due to his intense focus on catching. However, he was cursed with terrible throwing abilities (to almost impossible degrees, sometimes), making him fail to be on even the backup lineup for the club. When the baseball team explained that they needed balanced players, Monta is approached by Sena and decides to pursue his dream of becoming a master catcher as a football player instead.

The primary wide receiver of the Deimon Devil Bats. Although he is short in stature, he has remarkable skill in catching. He is observant, recognizing Sena as Eyeshield 21 the first time he saw Eyeshield 21 in person during a practice. He is best known for always going into a pose after a catch while yelling "CATCH MAX!" He also adds "Max" to nearly anything he describes while excited or determined. He is Hiruma's primary receiving target on the field and one of Sena's closest friends on the team. During the second game against Oujou, Raimon discovered his own special ability, the Devil Back-Fire, which his ability to predict Hiruma's passes due to trajectory and wind interference. This gives him the ability to rush forward at his target, keeping his attention focused and prepared for any tricks, then catch passes from behind him. As a former baseball player, he also has a talent for catching with one hand when necessary.

His nickname is Monta, which was initially a mistake by Sena but later accepted when Hiruma tells Taro that the name "Monta" is a play on the name Joe Montana (though Hiruma later admits that was just a fib).

Source:Wikipedia

Usually referred to as Hiruma, he is a protagonist and the major driving force of the plot of Eyeshield 21.
At the beginning of the series, he witnesses Sena Kobayakawa's running abilities and forces the boy to join the team as the ace running back and hides his identity during games by having him use the codename "Eyeshield 21".
He captains and plays quarterback for their team but also serves the duties of a coach, as the team lacks one. He is responsible for arranging all of the Devil Bats' matches between the Spring and Fall Tournaments and also devises the majority of the team's practice sessions.

Source:Wikipedia

Sena Kobayakawa a.k.a. Eyeshield 21 , is the main character.
Initially a bullied gopher, his speed was discovered by Hiruma as he was literally forced into joining the football team at Deimon. While insecure and clumsy at heart, Sena matures over the course of the series as he soon grows to love the game he plays and becomes an accomplished football player through his own strength and the guidance of his friends and rivals.

Source:Wikiepdia

Position: Center / Defensive Tackle / Long snapper

The largest and strongest player in the Deimon Devil Bats, Kurita is a kind-hearted individual and the only thing bigger than his body, is his heart.
He becomes Sena's second friend after entering Deimon High School. When playing, Kurita shouts his kiai (battle cry) "Funnuraba!" (Here I come!) when exerting extra effort. His biggest dream is to compete in the Christmas Bowl.

Yusuke Murata, the artist of the series, said that Kurita is his favorite character.

Source:Wikipedia