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Isshin Kurosaki (黒崎 一心, Kurosaki Isshin) is the father of Ichigo, Karin and Yuzu and head of the Kurosaki family. He runs their private clinic, the Kurosaki Clinic. A skilled doctor, Isshin can perform almost any medical procedure except major surgery.

While Isshin can be serious and grim at times, he typically acts in a goofy, energetic and fun-loving manner. He cares greatly for his children and takes a keen interest in what his daughters are doing, but less so with Ichigo, feeling that he does not need to be watched over as he views boys as different. He is willing to make a fool of himself in order to cheer up the kids and spends a great deal of time with the twins. Isshin is a big fan of Don Kanonji's TV show. As a Shinigami, Isshin is much more serious, but is still prone to arguing.